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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/12/2023 |
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Data da última atualização: |
13/12/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
LACORTE, C. C.; FERREIRA, A. L.; MURAD, A. M.; CUNHA, N. B. da; PINHEIRO, P. V. |
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Afiliação: |
CRISTIANO CASTRO LACORTE, Cenargen; AMANDA LOPES FERREIRA, bolsista CNPAF; ALINE MELRO MURAD, UFSC; NICOLAU BRITO DA CUNHA, UNB; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Seed-based production of recombinant proteins. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6. |
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Páginas: |
Cap. 6, p. 185-208. |
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DOI: |
https://doi.org/10.1007/978-981-99-4859-8_6 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues |
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Palavras-Chave: |
Foreign gene expression; Fusion proteins; Heterologous protein production; Plant molecular farming; Recombinant protein purification. |
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Thesaurus NAL: |
Biosafety; Glycosylation. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02273naa a2200277 a 4500
001 2159472
005 2023-12-13
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/978-981-99-4859-8_6$2DOI
100 1 $aLACORTE, C. C.
245 $aSeed-based production of recombinant proteins.$h[electronic resource]
260 $c2023
300 $aCap. 6, p. 185-208.
520 $aThe use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues
650 $aBiosafety
650 $aGlycosylation
653 $aForeign gene expression
653 $aFusion proteins
653 $aHeterologous protein production
653 $aPlant molecular farming
653 $aRecombinant protein purification
700 1 $aFERREIRA, A. L.
700 1 $aMURAD, A. M.
700 1 $aCUNHA, N. B. da
700 1 $aPINHEIRO, P. V.
773 $tIn: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6.
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